The sisters of Novo-Diveevo participate in a full-cycle of worship. Church services are held twice daily – mornings and evenings. In church the sisters read, sing, and manage the candle stand. They also read the Akathists and the Psalter daily with prayers for the living and the reposed.

Outside of the church, the sisters manage the sacristy, candle factory, baking prosfera, the convent archive, and the Book Nook. Among their many other responsibilities, they also engage in gardening and apiary care, as well as, baking unleavened bread for pilgrims.